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HPLC method for the determination in Radix Indirubin tablets


0,[link widoczny dla zalogowanych],3,6,[link widoczny dla zalogowanych],12,24,48 h were obtained solution measured peak area ,[link widoczny dla zalogowanych], the results show that the stability of 24h . 2.6 The average recovery test , accurately weighed sample ( batch number 981012 ) 6 copies , respectively, by adding different amounts of precision control indirubin solution method is determined by content of the sample , the average recovery was 100.9% , Northwest Pharmaceutical 1O Magazine January 2003 Vol 18 No. 5 203RSD 1.2. 2.7 Sample determination to take 2O films, precision set , small study ; accurately weighed small study sample 5.0g, add chloroform 100mL, Heated in a water bath reflux 60min, filter ; filtrate was concentrated post- 5mL volumetric flask,[link widoczny dla zalogowanych], dilute to the mark with chloroform ; injection 2OL, was chromatograms, shown in Figure 1. From the regression equation Indirubin ,[link widoczny dla zalogowanych], 3 batches of assay results in Table 1. 05l0l52O25t/minAa05l0l52025t/min lozenge I'IPLC Radix Figure 1 Figure A for a reference substance ; B tablets of a Radix ; a one indigo jade Red Table 13 tablets Isatis approved the determination of indirubin author discussed the results of 3 were used chloroform , ethanol as solvent, refluxed at different times , compare the content of indirubin , the results show that the sample was refluxed with chloroform more appropriate 60min .
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