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Kampo inhibit cancer liver metastasis characteristics of scavenging reactive oxygen prescription


.) A 15 to 19 reported Shiquandabutang, Si Wu Tang, Wen-ching, drink, Bu Zhong Yi Qi Tang in rats by oral administration can inhibit cancer metastasis, Shiquandabutang and activate Siwutang macrophages, NK cell activation can Buzhongyiqitang and so on. This study first analyzed Shiquandabutang Kampo prescriptions and their components such as crude drug scavenging reactive oxygen species (, · OH) characteristics, further analysis of activated macrophages and NK cells, characteristics of crude drugs, prescription of Kampo inhibit cancer liver metastasis mechanism. Materials and Methods: Shiquandabutang, Si Wu Tang, Decoction, Liujunzi Tang, Wen-ching, drink, ginseng Yang Rong Tang, Shaoyao San, Bu Zhong Yi Qi Tang, Xiao Chai Hu Tang 9 Kampo prescriptions and containing 23 crude drug extracts flavor of inhibition of reactive oxygen species. ① measured by ESR, including the activity of removal; ② SOD-like activity assay; ③ OH by ESR determination of the activity clear, · OH generation by Fenton reaction and ultraviolet (UV) irradiation 2 methods. Results: The study suggests that among students the role of prescription drugs and compare the concentration of 2.0mg/mL appropriate. ① clear activity: the highest rate of prescription in the inhibition of a four-Wu-Tang (73.3%), followed by Wenqingyin, Shaoyao San, Xiao Chai Hu Tang and so on. Berberine in crude drugs to the highest (92.1%), followed by baicalin, cassia twig, peony, etc., Ginseng and Pinellia no inhibition. Comparison of SOD-like effect, recipe Siwutang (16.5U/lnL), followed by Wenqingyin, Angelica and Peony Powder, Shiquandabutang, Xiao Chai Hu Tang: Health and Medicine in Coptis (68.2U/mL) activity the strongest, followed by other overseas Chinese medicine Medicine Volume 24 2002 Volume 4 Gui, Huang Qin,[link widoczny dla zalogowanych], peony, Cimicifuga. ② Clear OH activity: inhibition in Femon reaction Xiao Chai Hu Tang is the highest (57.0%), followed by Bu Zhong Yi Qi Tang, Si Wu Tang, Wen-ching,[link widoczny dla zalogowanych], drink, Sijunzitang, crude drug in Tuckahoe the highest (87.9%), followed Bupleurum, Ginger, Alisma, Schisandra, Astragalus, gardenia, ginseng, cohosh. Xiao Chai Hu Tang UV irradiation, the highest inhibition rate (54.9%), followed by Wenqingyin,[link widoczny dla zalogowanych], Liujunzi Tang, Bu Zhong Yi Qi Tang, Sijunzitang, crude drug in the baicalin (78.1%) the highest, later followed by Cork, Polygala, Cimicifuga, etc., Poria and ginger is negative. ③ the combined effects of clearance and OH: on and Fenton reaction, UV irradiation inhibition rate of the sum of 3, Xiao Chai Hu Tang was the highest (174.7%), followed by Wenqingyin, Bu Zhong Yi Qi Tang, Shiquandabu soup, ginseng Yang Rong Tang, Si Wu Tang. Pinellia the lowest (20.2%). Discussion: The results showed Shiquandabutang, Si Wu Tang, Wen-ching, clean drinking has a strong role, composition of crude drug to yellow, peony clear the role of strong, 3 has the composition of the prescription 4 Siwutang kinds of crude drugs have a stronger scavenging effect. SOD-like activity from the measured results can also see the same tendency. Bu Zhong Yi Qi Tang and Xiao Chai Hu Tang clear the role of OH intensity. Two formulas are the ginger, Bupleurum clear the role of OH intensity. With previous reports that students from the strong scavenging effects of the prescription drug component may be associated with the activity of macrophages, the role of OH by the clear and strong crude drug composition of the prescription may be related to NK cell activity. The results show that the Guizhi containing tannin, peony clear the role of strong,[link widoczny dla zalogowanych], but with the skullcap flavonoids, dried tangerine peel, licorice, astragalus, cork has a clear tendency of stronger OH. (Rong Hui Zhang Miaohai Excerpt high school)
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